Glycolysis is a critical process in both normal cellular function and diseases such as cancer. Cells readily switch between this energy producing pathway and mitochondrial respiration, depending on energy demand and nutrient availability. As such, knowing how much glycolysis is being used by cells at any moment in time is important for understanding the interaction between metabolic activity and cell function. When glycolysis proceeds to completion, lactate and protons are extruded from the cell. The Seahorse XF Glycolytic Rate Assay Kit measures the resulting acidification rates and accounts for mitochondrial acidification to deliver the most accurate acidification-based measurement available.
This tutorial describes a novel assay that measures immune cell activation from within minutes of activation throughout the fully developed activated phenotype in both a qualitative and quantitative manner. This provides a sensitive window of opportunity to identify and characterize attributes that can drive immune responses to desired outcomes.